Skip to main content
. 2010 Sep 14;285(48):37170–37177. doi: 10.1074/jbc.M110.157396

FIGURE 4.

FIGURE 4.

Effects of the overexpression of WT LGS and activated mutant LGS on glucokinase, GLUT2, glycogen phosphorylase, and phosphoenolpyruvate carboxykinase expression levels in rat liver. A, RT-PCR analysis of liver GK, GLUT2, GP, and PEPCK mRNA levels in livers of fed and fasted rats overexpressing β-gal; data are relative to the fed β-gal group. B, RT-PCR analysis in fasted overexpressing β-gal, WT LGS, or mutant LGS rats; data are relative to the fasted β-gal group of rats. C, RT-PCR analysis in fed overexpressing β-gal, WT LGS, or mutant LGS rats; data are relative to the fed β-gal group of rats. In all cases, relative expression levels were calculated with the 2ΔΔCt method using 18 S rRNA as endogenous control, and data represent the mean ± S.E. (error bars) of the following: seven fasted and seven fed β-gal-overexpressing rats; five fasted and six fed WT LGS-overexpressing rats; and five fasted and five fed mutant LGS-overexpressing rats. In A the asterisk indicates significant difference, with p < 0.05.