FIGURE 7.

High-fat diet fed AMPK β2 KO mice have reduced glucose uptake and increased levels of muscle ceramide. A, basal glucose disposal rate (GDR) during hyperinsulinemic-euglycemic clamp in HFD-fed WT and β2 KO mice (n = 5–6). B, 2-deoxyglucose uptake in isolated EDL muscles from chow and HFD-fed WT and β2 KO mice treated with or without insulin. C, diacylglycerol (DAG) and ceramide levels in tibialis anterior muscle from HFD-fed WT and β2 KO mice (n = 6). Akt (D) Thr³⁰⁸ and (E) Ser⁴⁷³ phosphorylation in isolated EDL muscles from chow and HFD-fed WT and β2 KO mice treated with or without insulin. Values are mean ± S.E., n = 7–13. *, p < 0.05 compared with wild type. #, p < 0.05 compared with chow diet.