FIGURE 2.

Effects of dialysis in the presence of DTT on the thermal stability of replicase. The replicase purified by the standard method was subjected to dialysis with or without 10 mm DTT in a buffer composed of 50 mm Tris-HCl, pH 7.8, 5 mm MgCl₂, 1 mm EDTA, and 250 mm NaCl. RNA replication rates before (gray bar) and after (black bar) the incubation at 37 °C for 2 min in the absence of template RNA and NTPs were plotted.