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Thermal stability of cysteine mutants of the β subunit. A, replication activity of each cysteine mutant of the β subunit in the cell lysates. Replication rates were measured before (gray bar) and after (black bar) incubation of the lysate at 37 °C for 20 min without addition of template RNA and NTPs. Replication rates relative to that of the wild-type before incubation were plotted. The proportions of residual replicase activity after the incubation are shown in B.
