TABLE 1.

Inactivation rate constants (k_inact) of the U- and S-form replicases

	kinact
	Free statea		Working stateb
	37 °C	25 °C	37 °C	25 °C
	min	min	min	min
S-form	0.009 ± 0.002	0.002 ± 0.002	0.019 ± 0.0003	0.0059 ± 0.0001
U-form	3.6 ± 0.1	0.095 ± 0.006	0.066 ± 0.0004	0.024 ± 0.0003

^a k_inact in the free state shows the inactivation rate constant in the absence of template RNA and NTPs. The values were obtained by curve fitting the data shown in Fig. 1B (U-form at 37 °C) and supplemental Figs. S2A (S-form at 37 °C) and S2B (both forms at 25 °C).

^b k_inact in the working state shows the inactivation rate constant in the presence of template RNA and NTPs. The values were obtained by curve fitting the data shown in Fig. 3.