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. 2010 Sep 20;285(48):37251–37262. doi: 10.1074/jbc.M110.142315

FIGURE 3.

FIGURE 3.

The C-terminal domain of ATF3 is essential for ethanol-induced GCK down-regulation and apoptosis. A, MIN6N8 cells were transfected with GFP-empty, GFP-ATF3, GFP-ATF3(ΔN), or GFP-ATF3(ΔC) vectors, and then Western blot was performed. C, non-treated control; E, ethanol-treated group. B, TUNEL assay. TUNEL-positive apoptosis cell numbers were quantified. *, p < 0.01; **, p < 0.05. C, after transfection with each vector, insulin content and ATP levels were measured. *, p < 0.05 in comparison with the corresponding untreated control Neo-vector groups. **, p < 0.01 in comparison with the corresponding ethanol-treated ATF3 overexpressing groups. All of the results were obtained from three independent experiments. CTL, control.