FIGURE 1.
Copper chelators and ethylene-binding activity in ETR1 expressed in yeast. A, yeast membranes isolated from yeast cells expressing ETR1 were analyzed for 14C2H4 bound in the presence of various copper chelators in the presence or absence of 300 μm copper sulfate. The copper chelators diethyldithiocarbamate, bathocuproinedisulfonic acid, and neocuproine were tested at a concentration of 5 mm. B, data in the absence of added copper from A are shown at an expanded scale. Saturable ethylene binding to ETR1 was determined as described under “Experimental Procedures” and normalized to the amount of radioactivity in the presence of copper with no chelator added. The mean ± S.D. is shown.