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. 2010 Sep 27;285(48):37263–37270. doi: 10.1074/jbc.M110.170027

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Effects of copper chelators on the ran1-1 and ran1-2 mutants. A, the ethylene dose responses on hypocotyl length in wild-type (Columbia (Col)) and ran1 mutants are shown. Etiolated Arabidopsis seedlings were treated with the indicated concentrations of ethylene. B, the effects of 10 μm neocuproine and 100 μm bathocuproinedisulfonic acid on etiolated seedlings are shown. For comparison, seedlings not treated with a copper chelator are shown. The ran1 mutants are compared with their wild-type controls (Columbia for ran1-1 and ran1-2 and Wassilewskija (Ws) for ran1-3 and ran1-4). C, the dose response for neocuproine on the growth of hypocotyls of etiolated seedlings is shown. In all panels, seedlings were grown in the dark for 4 days under the conditions indicated. In A and C, data represent the mean ± S.D. for hypocotyl length.