FIGURE 2.

Role of the NB in the subcellular localization of Mycd. A, schematic representation of full-length and mutant Mycds is shown. Sequences of the NB (red letters) in Mycd and MRTF-A/B orthologs from different species are shown (right column). SAP, SAP domain; CC, coiled-coil domain. B, VSMCs were transfected with expression plasmids for the indicated FLAG-tagged Mycd derivatives and cultured under serum-stimulated conditions for 24 h. The cells were stained with an anti-FLAG antibody (red) and Hoechst 33258 (blue). Representative images from at least three independent experiments are shown. Bar = 10 μm. C, the images were quantified as described under “Experimental Procedures.” N, nuclear-specific accumulation; NC, diffuse distribution in the nucleus and the cytoplasm; C, cytoplasmic localization.