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. 2010 Sep 16;285(48):37314–37323. doi: 10.1074/jbc.M110.180786

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Interaction between Mycd family members and importin α/β1 heterodimers. A, mixtures of in vitro translated FLAG-Mycd, importin β1, and each of the indicated HA-importin α subtypes were immunoprecipitated with a control gel or anti-HA affinity gel, and the immunoprecipitates (IP) were analyzed by immunoblotting (IB) using the indicated antibodies (Ab). B and C, in vitro translated FLAG-Mycd, its derivatives, FLAG-MRTF-A, or FLAG-MRTF-B was mixed with importin β1 and HA-importin α1, and their interaction was analyzed. The upper and lower panels show input and immunoprecipitation, respectively. Representative results from at least three independent experiments are shown.