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Determination of the orientation of the hydrophilic loops of DGAT1 by trypsin digestion. Total membranes were prepared from HEK-293T cells expressing FL-DGAT1-Myc with internal HA tags (A) or FL-DGAT1-HA (B) with internal Myc tags. Protease protection assays were performed as described in Fig. 3. Samples were analyzed by immunoblotting with anti-FLAG, anti-Myc, anti-HA, or anti-PDI antibodies.
