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. 2010 Sep 27;285(48):37377–37387. doi: 10.1074/jbc.M110.163691

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — The N terminus of DGAT1 has a role in oligomer formation. Total membranes isolated from HEK-293T cells expressing (A) FL-DGAT1, FL-DGAT1(Δ2–34), and FL-DGAT1(Δ2–95) were incubated in the (−) absence or (+) presence of 1 mm DSS. After the cross-linking reactions were terminated, samples were subjected to SDS-PAGE and immunoblotted with anti-FLAG. 1×, 2×, and 4× refer to DGAT1 monomer, dimer, and tetramer, respectively. B, after cross-linking with 1 mm DSS, samples were treated with or without trypsin and immunoblotted with anti-FLAG, anti-Myc, and anti HA antibodies. Oligomers of the cross-linked 16-kDa protected fragment recognized by anti-HA (*). An oligomer of unknown composition is indicated by ?.