FIGURE 1.

TE is modified by reactive oxygen and nitrogen species. Recombinant bovine TE (1 μg) was exposed to indicated concentrations of ONOO⁻ (A and B), HOCl (C), or H₂O₂ (D) and subjected to immunoblot analyses for nitrosative modifications using an anti-nitrotyrosine (N-Tyr) antibody (A) and oxidative modifications using OxyBlot protein oxidation detection kit (B–D). The same membranes were also probed with an anti-elastin antibody to control for loading. Data are representative of at least three independent experiments.