FIGURE 6.

Oxidative modification of TE reduced binding to Fbn-2 but not to MAGP1. A 96-well non-tissue culture plates coated with 1 μg/well of unmodified (UnOx-TE, closed circle) or ONOO⁻-modified (Ox-TE, open circle) were incubated with indicated concentrations of Fbn-2 fragment (A) or MAGP1 (B) at 37 °C for 3 h. Following washing, bound Fbn-2 or MAGP1 was detected using an anti-Gly antibody for Fbn-2 or anti-MAGP1 antibody, HRP-conjugated secondary antibody, and the ABTS Peroxidase Substrate System, and measuring the absorbance at 410 nm. Data represent the mean of at least three independent experiments done in duplicate ± S.E. *, p < 0.05; **, p < 0.005 compared with unmodified TE.