Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 28;285(48):37405–37414. doi: 10.1074/jbc.M110.149468

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Secreted IDE is associated with exosomes. A, conditioned cell culture media obtained from control and lovastatin (5 μm)-treated BV-2 cells was subjected to subsequent centrifugation steps. The indicated proteins were detected in the respective pellets (P, upper panel) and supernatants (S, lower panel). B, characterization of proteins in exosome preparations from conditioned cell culture media of control and lovastatin-treated (5 μm, 24 h) BV-2 cells. Total cell lysates served as controls. C, co-fractionation of exosomal proteins and IDE. The P100 fraction of conditioned media from lovastatin-treated BV-2 cells (see A) was separated in a sucrose gradient by ultracentrifugation. Resulting fractions were analyzed for IDE, alix, and flotillin-1. D, electron micrographs of P100 fractions. Purified exosomes from conditioned media of statin-treated BV-2 cells were resuspended in 2% paraformaldehyde and negatively stained with uranyl acetate (scale bars, 200 nm). E, quantitative analysis of vesicle sizes in the P100 fraction. The distribution of mean diameter of vesicles was analyzed by scoring 10 randomly selected electron micrographs.