FIGURE 5.
RANK IVVY535–538 motif plays a critical role in TNF-mediated osteoclastogenesis. A, schematic diagram of Ch1 and Ch2. B, flow cytometric analysis of BMMs infected with virus encoding GFP, Ch1, or Ch2. C, BMMs expressing GFP, Ch1, or Ch2 were treated with M-CSF (M, 44 ng/ml) and Fas-AB (F, 100 ng/ml) for 18 h. The cultures were then continued with M-CSF (44 ng/ml) alone or M-CSF (44 ng/ml) and TNF (T, 5 ng/ml) for 4 days (d). Infected BMMs treated with M-CSF (44 ng/ml) plus Fas-AB (100 ng/ml) or M-CSF (44 ng/ml) plus TNF (T, 5 ng/ml) for 5 days served as positive and negative controls, respectively. The cultures were then stained for TRAP activity.