FIGURE 7.
TNF-induced osteoclastogenesis attended by permissive Fas-AB dosage requires RANK IVVY535–538-mediated signaling. A, flow cytometric analysis of BMMs infected with virus encoding GFP, Ch1, or Ch2 is shown. B, BMMs expressing GFP, Ch1, or Ch2 were treated with M-CSF (M, 44 ng/ml) and Fas-AB (F, 10 ng/ml) with or without TNF (T, 5 ng/ml) for 5 days (d). The cultures were then stained for TRAP activity. C, BMMs expressing GFP, Ch1, or Ch2 were treated with M-CSF (44 ng/ml) and Fas-AB (10 ng/ml), M-CSF (44 ng/ml) and TNF (5 ng/ml), or M-CSF (44 ng/ml) + Fas-AB (10 ng/ml) + TNF (5 ng/ml) for 3 days. Gene expression was determined by semiquantitative RT-PCR.