FIGURE 3.

Release of calcein from artificial liposomes. The ability of peptides to disrupt artificial liposomes was measured using a calcein release assay. NaD1 and Bac2A failed to release calcein from liposomes composed of PC/PG (3:1; black bars), PC/PE/PS/PI (5:3:1:1; white bars), or PC/PE/PS/PI/ergosterol (5:3:1:1:0.1; gray bars). CP-29 and BMAP-28 induced substantial calcein release from all three liposomes, whereas LL-37 was more effective against PC/PG liposomes but was still able to disrupt PC/PE/PS/PI liposomes. Percent calcein release is relative to Triton-X-100 (0.1%), and error bars represent S.E. (n = 4). All peptides were used at 20 μm.