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Killing of hyphal cells after cell wall treatments. The death of untreated fungal hyphae (black bars) was compared with that of hyphae that had been treated with either proteinase K (gray bars) or β-glucanase (white bars). Both proteinase K and β-glucanase treatments prevented NaD1-induced cell death. In contrast, CP-29 and Bac2A killed treated hyphae as efficiently as the untreated control. All peptides were used at 5 μm, and error bars represent S.E. (n = 4).
