FIGURE 5.

Effect of iodoacetate on the dynamics of NADH and intracellular ATP in yeast cells with an oscillating glycolysis. Time-resolved NADH fluorescence, ATP, and ADP concentrations before and after addition of 20 mm iodoacetate to the yeast suspension. Iodoacetate was added at the indicated arrow. The ADP concentration shown as a dashed curve was determined using Equation 4 instead of Equation 3, again assuming a total concentration of ADP and ATP ([A]₀) of 3.6 mm. Experimental conditions are as described in the legend of Fig. 2.