FIGURE 6.

Effect of 2-DG on the dynamics of NADH and intracellular ATP following addition of glucose and KCN to a suspension of yeast cells. The cells were incubated with 5 mm 2-DG for 10 min before addition of 30 mm glucose and 5 mm KCN as indicated on the graph. The point at time t = 2400 s and standard deviations (n = 3) in the ATP graph represents an off-line measurement of intracellular ATP made by quenching the cells and measuring the ATP content of the cell extract using a luciferase assay. Experimental conditions are as described in the legend of Fig. 2.