FIGURE 2.

Effects of fluid shear on [³H]thymidine incorporation (A) and Erk1/2 phosphorylation (B) of osteoblasts of young adult female B6 mice, wild-type littermates of ob⁻/ob⁻ mice (WT ob⁺/ob⁺), or leptin-deficient (ob⁻/ob⁻) mice. In A, results are shown as the percentage of respective static cells (mean ± S.D. (error bars), n = 4). *, p < 0.05 versus static control; #, p < 0.05 versus the B6 and WT cells. In B, the top shows a representative blot of pErk1 and pErk2 (using an anti-pErk1/2 antibody) and total Erk1/2 (identified by an anti-pan-Erk antibody). The bottom summarizes the relative amounts of pErk1/2 normalized against total Erk, and results are shown as a percentage of respective static control (mean ± S.D., n = 4). *, p < 0.05, compared with static control; #, p < 0.05, compared with B6 osteoblasts or WT osteoblasts.