FIGURE 7.

Suppression of Lepr expression in C3H osteoblasts by a 24-h treatment with 5 nmLepr siRNA2 restored their mitogenic response to fluid shear stress. Primary osteoblasts isolated from 10-week-old female C3H mice were treated for 24 h with 5 nm Lepr siRNA2 (right bars) or the control siRNA (left bars). After the respective siRNA treatment, the cells were placed in the flow chamber with (dark bars) or without (open bars) subjection to a 30-min shear stress of 20 dynes/cm². Cell proliferation was measured by [³H]thymidine incorporation during the final 6 h of a 24-h incubation. Results are shown as mean ± S.E. (error bars) (n = 4). N.S., not significant.