. 2010 Sep 17;285(48):37607–37618. doi: 10.1074/jbc.M110.169714

TABLE 1.

Effect of an overnight pretreatment with 100 ng/ml leptin on the shear stress-induced expression of genes of the IGF-I, BMP/TGFβ, ER, and Wnt signaling pathways in B6 osteoblasts and ob⁻/ob⁻ osteoblasts by real-time PCR 4 h after the fluid shear (n = 6 for each)

Gene	B6 osteoblasts		ob⁻/ob⁻ osteoblasts
Gene	Stressed/Static control (no leptin, -fold changes, mean ± S.D.)	Stressed/Static control (with leptin, -fold changes, mean ± S.D.)	Stressed/Static control (no leptin, -fold changes, mean ± S.D.)	Stressed/Static control (with leptin, -fold changes, mean ± S.D.)
	-fold	-fold	-fold	-fold
Era	2.40 ± 0.75^a	1.39 ± 0.25^b	3.31 ± 0.20^a	1.18 ± 0.24^b
Igf1r	3.30 ± 0.36^a	1.55 ± 0.29^b	4.49 ± 0.71^a	1.78 ± 0.38^b
Dlx1	1.68 ± 0.48^a	0.75 ± 0.10^b	3.23 ± 0.65^a	1.12 ± 0.21^b
Ncoa1	2.89 ± 0.11^a	1.34 ± 0.60^b	3.74 ± 0.88^a	0.98 ± 0.17^b
c-fos	3.06 ± 0.68^a	1.25 ± 0.22^b	2.56 ± 0.29^a	1.32 ± 0.08^b
Ctnnb1	2.62 ± 0.54^a	0.90 ± 0.30^b	3.80 ± 0.62^a	1.08 ± 0.28^b
Wnt1	2.71 ± 0.74^a	0.93 ± 0.09^b	3.65 ± 0.52^a	1.68 ± 0.34^b
Wnt3a	2.56 ± 0.75^a	1.66 ± 0.25^b	3.00 ± 0.62^a	1.52 ± 0.18^b

^a p < 0.05, compared with the corresponding static control.

^b p < 0.05, compared with those without the leptin pretreatment.