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. 2010 Sep 14;285(48):37683–37692. doi: 10.1074/jbc.M110.162735

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Comparison of mucin-type O-glycans by mass spectrometric analyses of proliferating and differentiated Caco-2 cells. MALDI-MS analysis of permethylated O-glycans in positive ion mode from proliferating and differentiated Caco-2 cells. 30–40% confluent Caco-2 cells served as proliferating cells. At both 3 and 13 days after confluency, Caco-2 cells were assessed as differentiated and further differentiated cells. The m/z values of the labeled peaks correspond to monoisotopic mass, and assignment of the molecular composition is shown in the figure. Ions at m/z 983.5, 1157.6, 1344.7, 1518.8, 1705.9, and 1800.0 correspond to core3 O-glycans, Gal→GlcNAc→(Gal→)GalNAcitol, Gal→(Fuc→)GlcNAc→(Gal→)GalNAcitol, Gal→GlcNAc→(Sialic acid→Gal→)GalNAcitol, Gal→(Fuc→)GlcNAc→(sialic acid→Gal→)GalNAcitol, (sialic acid)2→Gal→GlcNAc→(Gal→)GalNAcitol, and (sialic acid)2→Gal→(Fuc→)GlcNAc→(Gal→)GalNAcitol, respectively.