FIGURE 4.

Maturation of SI is attenuated in C2GnT-2-deficient mice. Expression of SI was compared from two different intestinal tissue samples from wild-type and C2GnT-2-deficient mice (A). Attenuated maturation was seen only in C2GnT-2-deficient and not in C2GnT-3-deficient mice (B). Four mg of each tissue sample was homogenized, and the resulting supernatant was used in Western blotting. Anti-goat SI antibody was used in this experiment as described under “Experimental and Procedures.”