Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 17;285(48):37704–37715. doi: 10.1074/jbc.M110.140517

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 7. — Cell surface heparan sulfates have an inhibitory effect on FGFRL1-mediated cell fusion. CHO-K1 cells, CHO-677 cells (lack heparan sulfates) and CHO-PgsA cells (lack all glycosaminoglycans) were seeded together with FGFRL1ΔC-transfected HEK-TetOn cells and left to fuse overnight. A, representative pictures of the resulting HEK/CHO syncytia are shown for each CHO cell line. Bar, 100 μm. The diagram gives the average number of nuclei per syncytium of the fusing CHO-K1, CHO-677, and CHO-PgsA cells with FGFRL1-transfected HEK cells. Both CHO-677 and CHO-PgsA cells displayed dramatically increased cell fusion efficiency when compared with the wild-type CHO-K1 cells, indicating that heparan sulfates have an inhibitory effect on FGFRL1-induced cell-cell fusion. B, luciferase (pTRE-Luc)-transfected CHO-K1 and FGFRL1 wild-type, FGFRL1ΔC-, and FGFRL1ΔCΔ12-transfected HEK-TetOn cells were co-cultured overnight in the absence and presence of exogenous heparin or chondroitin-4-sulfate in the culture medium, followed by luciferase measurement.