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Activity and nucleotide binding of C. glutamicum PimB′ and of selected point mutants. A, analysis of transfer of 14C-labeled mannose from GDP-[14C]Man to acylated phosphatidylinositol mannoside by scintillation counting and thin layer chromatography. B, saturation binding of GDP-Man or GDP to PimB′ monitored by internal Trp fluorescence. C, far UV CD spectra of wild-type PimB′ and of the H118S mutant.
