TABLE 2.

Activity and saturation binding for PimB′ and selected point mutants

	GDP-Man Kd	GDP Kd	Activity	Residual Activity
	μm	μm	cpm	%
WT	19.0 ± 4.6	36.5 ± 6.9	5528 ± 979	100.0
Blank	NAa	NA	30 ± 22	0.5
N12A	20.4 ± 6.2	21.3 ± 5.5	1723 ± 77	31.2
D13N	30.1 ± 6.9	18.8 ± 5.1	83 ± 5	1.5
D13Y	NA	NA	16 ± 0	0.3
D13A	42.8 ± 11.9	61.3 ± 17.4	104 ± 5	1.9
G20W	21.9 ± 6.4	46.5 ± 40.2	176 ± 15	3.2
I21S	13.4 ± 7.0	26.5 ± 7.4	134 ± 22	2.4
I21A	56.0 ± 14.5	40.9 ± 8.6	110 ± 5	2.0
Q22A	60.3 ± 22.8	30.6 ± 6.4	788 ± 218	14.3
H118S	72.4 ± 27.5	19.6 ± 9.8	36 ± 3	0.7
H120S	NA	NA	4490 ± 524	81.2
G123P	29.3 ± 5.9	42.3 ± 9.3	4784 ± 298	86.6
S205G	18.7 ± 4.6	33.6 ± 6.9	6609 ± 1207	119.6
R206S	42.7 ± 9.4	72.4 ± 17.2	311 ± 47	5.6
R210S	40.2 ± 10.7	44.1 ± 28.8	481 ± 58	8.7
K211Q	30.5 ± 17.6	40.3 ± 14.6	193 ± 25	3.5
E290Q	35.7 ± 18.9	41.7 ± 21.5	252 ± 30	4.6
E290D	55.0 ± 17.7	322.7 ± 518.7	162 ± 14	2.9
E290N	NBb		59 ± 16	1.1
G291S	68.7 ± 35.5	74.2 ± 37.2	292 ± 241	5.3

^a NA, binding not assayed.

^b NB, no binding detected.