FIGURE 8.
Thermal stability of p53 serine 215/269 substitution mutants. A, thermal melting profile of p53 mutants. Unfolding of p53 mutants was monitored between 15 and 55 °C using SYPRO Orange fluorescent dye, and melt curves were generated by plotting SYPRO Orange fluorescence as a function of temperature. B, phase transitions in the thermal melting profile. The gradient of p53 unfolding was plotted as a function of temperature to obtain the midpoint temperature transition (Tm) for each variant. Midpoint transition temperatures for each mutant are indicated. RFU, relative fluorescent units.