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. 2010 Sep 20;285(48):37797–37810. doi: 10.1074/jbc.M110.169086

FIGURE 8.

FIGURE 8.

The three conserved EREs are essential and cooperative for full HOXC4 promoter activation. A, spleen B cells from HoxC4+/+ female mice were transfected with pGL3-enhancer luciferase gene reporter constructs containing unmutated (unMut) or mutated HOXC4 promoter in which all three of the EREs were deleted (ERE-1-2-3 Mut). The transfected B cells were stimulated with LPS and rmIL-4 in the presence or absence of E2 (10 nm), and luciferase activity was measured 24 h thereafter. Data are from three independent experiments (mean ± S.D. (error bars)). B, human 2E2 B cells were transfected with pGL3-enhancer luciferase gene reporter constructs containing unmutated or mutated HOXC4 promoter in which one (ERE-1 Mut, ERE-2 Mut, or ERE-3 Mut) or three (ERE-1-2-3 Mut) of the EREs were deleted. The transfected B cells were stimulated with nil, E2 (10 nm) alone, or anti-CD40 mAb and rhIL-4 in the presence or absence of E2 (10 nm), and luciferase activity was measured 24 h thereafter. Data are from three independent experiments (mean ± S.D.).