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. 2010 Apr;62(2):192–202. doi: 10.1111/j.1365-313X.2010.04138.x

Figure 5.

Figure 5

Site-directed phosphomutants are differentially affected in plastid gene expression. Northern blot analyses were carried out with total cellular RNA (1 μg each) from the wild type (WT), the AtSig6 knock-out line (sig6-2) and the retransformed lines, including those containing fully functional AtSIG6 cDNA (sig6-com) and various point mutant derivatives. Hybridization was performed using DIG-labelled RNA probes for atpB, psbA and a nuclear control gene (actin2), with RNA from 7-day-old seedlings (7d) and 14-day-old plantlets (14d). Transcript sizes (kb) are indicated in the right margin.