Table 1.
Position in consensus motif | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Position | Aminoacid | NetPhos 2.0 | NetPhosK 1.0 | KinasePhos 2.0 | disphos | gps | PREDPhospho | ScanSite | −2 | −1 | * | +1 | +2 | +3 | +4 | Homology |
26 | Serine | * | * | Y | S | S | P | S | S | V | ||||||
94 | Serine | * | * | * | * | * | * | L | V | S | S | R | E | D | ||
95 | Serine | * | * | * | * | * | * | V | S | S | R | E | D | E | ||
149 | Serine | * | A | L | S | A | S | K | Q | |||||||
174 | Serine | * | * | * | * | S | L | S | T | S | S | S | AtSIG2 | |||
176 | Serine | * | * | * | S | T | S | S | S | M | S | |||||
180 | Serine | * | * | * | * | * | S | M | S | L | P | E | K | AtSIG5 | ||
206 | Serine | * | P | K | S | N | D | V | D | |||||||
244 | Threonine | * | P | E | T | K | Q | L | L | |||||||
249 | Threonine | * | L | L | T | A | K | E | E | AtSIG1-5 | ||||||
282 | Threonine | * | E | P | T | I | G | E | W | |||||||
306 | Serine | * | * | G | R | S | S | R | E | K | ||||||
411 | Serine | * | * | * | R | P | S | K | E | E | L | AtSIG4, 5 | ||||
423 | Threonine | * | V | S | T | E | K | L | D | |||||||
445 | Serine | * | * | I | W | S | D | Q | D | T | ||||||
450 | Threonine | * | * | D | T | T | F | Q | E | I | AtSIG4 | |||||
458 | Serine | * | * | * | * | P | D | S | G | I | E | T | AtSIG1, 3, 5 | |||
462 | Threonine | * | I | E | T | P | T | M | S | AtSIG2, 3, 4 | ||||||
484 | Serine | * | * | * | V | L | S | P | K | E | R | AtSIG4 | ||||
504 | Serine | * | * | * | Q | R | S | L | S | E | I | AtSIG1-5 | ||||
CK2 consensus substrate site | S/T | x | x | E/D | x |
Prediction tools NetPhos 2.0 (Blom et al., 1999), NetPhosK 1.0 (Blom et al., 2004), KinasePhos 2.0 (Wong et al., 2007), disphos (Diella et al., 2004), gps (Xue et al., 2005), PREDPhospho (Kim et al., 2004) and ScanSite (Obenauer et al., 2003) were used, leading to the detection of putative sites (+). Those selected for subsequent experiments are set in bold. The phosphoacceptor residue (*) and the acidic residue at the n + 3 position are marked in bold. Arabidopsis sigma factors showing regional similarity with AtSIG6 CK2 sites are indicated in the last column (Figure S2 for detailed positions). Bottom: consensus sequence of CK2 phosphorylation sites (Meggio and Pinna, 2003).