Figure 3.

Distribution of TLR expression in different LP DC subsets. (A) TLR are expressed mainly by LP DC (CD11c⁺CD11b^–F4/80^–). CD11c⁺TLR2^– and CD11c⁺TLR2⁺ subsets of freshly-isolated LP cells were shown in the top left panel. The proportions of live-gated CD11c⁺TLR2^– (black) and CD11c⁺TLR2⁺ (grey) DC expressing surface F4/80 or CD11b are shown in the top right panel. CD11c⁺TLR4^– and CD11c⁺TLR4⁺ subsets of freshly-isolated LP cells were shown in the bottom left panel. The proportions of live-gated CD11c⁺TLR4^– (black) and CD11c⁺TLR4⁺ (grey) DC expressing surface F4/80 or CD11b are shown in the bottom right panel. (B) Expression of TLR by the CD11c⁺B220^– and CD11c⁺B220⁺ DC subsets within the small intestinal LP cells (left panel). The proportions of live-gated CD11c⁺B220⁺ (black) and CD11c⁺B220^– (grey) DC expressing intracellular TLR3 or TLR9 are shown (right panel). (C) Two subpopulation of DC CD11c⁺CD103⁺ (grey) and CD11c⁺ CD103^– (black) were found in freshly isolated small intestinal LP cells (left panel). The percentages of gated CD11c⁺CD103⁺ (grey) and CD11c⁺CD103^– (black) DC expressing intracellular TLR9 or surface TLR3 are shown (right panel). The data shown are the percentages of F4/80⁺, CD11b⁺ or TLR⁺ cells out of live-gated (PI^–) CD11c⁺ cells in each tissue and are the mean + 1 SD of three separate experiments.