Skip to main content
. 2010 Jun 21;63(4):599–612. doi: 10.1111/j.1365-313X.2010.04264.x

Figure 6.

Figure 6

Regulation of diterpenoid phytoalexin biosynthesis by OsMKK4DD.(a) The isoprenoid synthesis and diterpenoid metabolism pathways, showing the enzymes whose genes were up- or down-regulated by OsMKK4DD in wild-type (WT) cells. The numbers indicate gene expression ratios (OsMKK4DD versus vector control) at 12 h after the beginning of dexamethasone (DEX) treatment. Red and blue characters indicate up- and down-regulation, respectively. The asterisks indicate the significance levels according to the one-sample t-test (*P<0.05, **P<0.01).(b) Bar graphs of gene expression ratios derived from the microarray data set for osmpk6 and osmpk6/OsMPK6 cells expressing OsMKK4DD (+DEX) compared with the same cells treated with ethanol (–DEX) for 12 h.(c), (d) Levels of momilactone A and B (c) and phytocassane A–E (d) in cells after treatment with 10 μm DEX. Two independent cell lines were used for each construct and three independent experiments were performed for each line, with reproducible results. The graphs represent the means of data from a representative experiment, and the error bars indicate standard deviations.Abbreviations: AACT, acetoacetyl-CoA thiolase; CMK, 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase; CMS, 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol synthase; CPP, copalyl diphosphate; CPS, copalyl diphosphate synthase; CRTISO, carotenoid isomerase; DMAPP, dimethylallyl diphosphate; DXP, 1-deoxy-d-xylulose 5-phosphate; DXR, DXP reductoisomerase; DXS, DXP synthase; FPP, farnesyl diphosphate; FPPS, FPP synthase; GGDP, geranylgeranyl diphosphate; GGR, geranylgeranyl reductase; GGPS, geranylgeranyl diphosphate synthase; GPP, geranyl diphosphate; GPPS, geranyl diphosphate synthase; HDS, HMBDP synthase; HDR, HMBDP reductase; HMBDP, 1-hydroxy-2-methyl-2-(E)-butenyl4-diphosphate; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; IPI, isopentenyl diphosphate isomerase; IPP, isopentenyl diphosphate; KAO, ent-kaurenoic acid oxidase; KO, ent-kaurene oxidase; KS, kaurene synthase; MAS, momilactone A synthase; MCS, 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase; MK, mevalonate kinase; MPDC, mevalonate diphosphate decarboxylase; PDS, phytoene desaturase; PMK, phosphomevalonate kinase; PS, phytoene synthase; SQLE, squalene epoxidase; SQS, squalene synthase; TPS, monoterpene/sesquiterpene synthase-like genes; ZDS, zeta-carotene desaturase.