Table 1.
Sequenced deletions and rearrangements arising in the HSV-tk gene that involve the telomeric repeat region after replication in human cells
| Mutation Class | Telomeric Insertsa | ||||
|---|---|---|---|---|---|
| No insert | [CCCTAA]10 | [TTAGGG]10 | [TTGGGG]10 | [GGGGTTTT]5 | |
| Deletions and Rearrangements | |||||
| Median mutation rate | 13×10−7 | 5.6×10−7 | 7.6×10−7 | 18×10−7 | 1.5×10−7 |
| Proportion of total mutations | 0.39 | 0.15 | 0.33 | 0.24 | 0.030 |
| Number | 13 | 6 | 10 | 9 | 1 |
| Loss of repeats (proportion)b | 6 (0.46)c | 3 (0.50) | 5 (0.50) | 4 (0.44) | 0 |
| Retain repeats (proportion)b | 7 (0.54) | 3 (0.50) | 5 (0.50) | 5 (0.56) | 1 |
Values were derived from sequenced HSV-tk mutants in Supplemental Tables S6-S10. Small deletio ns (≤ 12 bp) were excluded. Identical mutants from a single clone were counted once to avoid including potential siblings.
a
DNA sequence of the lagging strand of replication
b
The proportion is based on the number of deletions and rearrangements from line 3.
c
Deletions or rearrangements that involved loss of base pairs 111 and 112. This is the region in which telomeric repeats were inserted for the other vectors (see Figure 2).