Skip to main content
. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: DNA Repair (Amst). 2010 Aug 25;9(11):1119–1129. doi: 10.1016/j.dnarep.2010.07.014

Table 1.

Sequenced deletions and rearrangements arising in the HSV-tk gene that involve the telomeric repeat region after replication in human cells

Mutation Class Telomeric Insertsa
No insert [CCCTAA]10 [TTAGGG]10 [TTGGGG]10 [GGGGTTTT]5
Deletions and Rearrangements
 Median mutation rate 13×10−7 5.6×10−7 7.6×10−7 18×10−7 1.5×10−7
 Proportion of total mutations 0.39 0.15 0.33 0.24 0.030
 Number 13 6 10 9 1
Loss of repeats (proportion)b 6 (0.46)c 3 (0.50) 5 (0.50) 4 (0.44) 0
 Retain repeats (proportion)b 7 (0.54) 3 (0.50) 5 (0.50) 5 (0.56) 1

Values were derived from sequenced HSV-tk mutants in Supplemental Tables S6-S10. Small deletio ns (≤ 12 bp) were excluded. Identical mutants from a single clone were counted once to avoid including potential siblings.

a

DNA sequence of the lagging strand of replication

b

The proportion is based on the number of deletions and rearrangements from line 3.

c

Deletions or rearrangements that involved loss of base pairs 111 and 112. This is the region in which telomeric repeats were inserted for the other vectors (see Figure 2).