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Membrane-bound NA-chIL-2 expressed on the surface of MDCK subclones is bioactive. Subclones 4, 15, and 33 of MDCK cells stably transfected with the NA-chIL-2 construct or wild-type (wt) MDCK cells were assayed for chicken IL-2 bioactivity by co-culturing mitomycin C pre-treated cells with activated chicken T blasts and measuring 3H-thymidine uptake (quadruplicate samples).
