FIG. 2.
Anti-chIL-2 antibodies neutralize membrane-bound chIL-2 bioactivity on MDCK/NA-chIL-2 cells. Three concentrations of rabbit anti-chIL-2 antiserum (A), monoclonal mouse anti-chIL-2 antibody O (specific for the C-terminus of chIL-2) (B) and monoclonal mouse anti-chIL-2 G (C) were evaluated for their neutralizing activity in an IL-2 bioassay. β-propiolactone treated MDCK/NA-chIL-2 cells seeded in 96-well plates were incubated with chicken T-cell blasts for 24 h in the presence of chIL-2 specific antibodies or nonspecific control antisera. Proliferation was measured by 3H-thymidine incorporation (quadruplicate samples), which was present during the final 6 h.