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. 2009 Jan;29(1):9–22. doi: 10.1089/jir.2008.0017

FIG. 9.

FIG. 9.

Virion-bound chIL-2 augments antibody responses in chickens. Chicks were injected twice with UV-inactivated filamentous influenza, A/Udorn, bearing chicken IL-2 (IVAC-chIL-2) or with wild-type influenza vaccine (IVAC); 10 μg viral protein per dose for chicks receiving an aqueous vaccine and 3 μg per dose for chicks receiving an oil emulsion vaccine. Sera were tested for virus-specific antibody 2 weeks after the boost by ELISA (7–9 chicks per group). Chicks injected with IVAC-chIL-2 (in a saline formulation) had significantly higher geometric mean titers than chicks injected with wild-type IVAC (ANOVA and Bonferoni's multiple comparison test; p < 0.05). Chicks receiving IVAC-chIL-2 in oil also had elevated antibody responses, but the difference was not statistically significant (p > 0.05).