VEGF secretion in response to in vitro treatment with IFN-α, IL-12, or the combination. MEL 39 and 18105 MEL cells (5 × 105 cells/condition) were treated with PBS, IFN-α (2000 U/mL), IL-12 (10 ng/mL), or IFN-α and IL-12 for 48 h, and VEGF secretion was assessed by ELISA.