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. 2008 Nov;28(11):667–678. doi: 10.1089/jir.2008.0023

FIG. 2.

FIG. 2.

FIG. 2.

Sp100A dimerization and EBNA-LP interaction domains are between amino acid residues 46–150. (A) Schematic representation of wild-type and mutant Sp100A. Boundaries of each deletion are indicated. (B) EBV-negative DG75 cells were cotransfected with 1–182 Sp100-Flag and wild-type or mutant Sp100A plasmids represented in (A). Following immunoprecipitation with anti-Flag antibodies, coprecipitated Sp100A was detected by Western blot using anti-HA antibodies. Anti-Flag antibodies were used to confirm 1–182-Flag precipitation. (C) Western blot detection of wild-type and mutant Sp100A proteins expressed in transfected DG75 cells. (D) DG75 cells were cotransfected with wild-type or mutant Sp100A and flag-tagged EBNA-LP expression plasmids. Immunoprecipitations and Western blots were carried out as outlined above, using anti-Flag and anti-HA antibodies.