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The Sp100A PML NB targeting domain is between amino acid residues 46–150. Immunofluorescence detection of transiently expressed Sp100A proteins in DG75 cells. Sp100A (red) was monitored using mouse monoclonal anti-HA antibodies. Cells were co-stained with DAPI to detect the nucleus. Merged panels are shown in the far right hand column as indicated.
