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. 2010 Oct 13;30(41):13707–13717. doi: 10.1523/JNEUROSCI.4040-10.2010

Figure 4.

Figure 4.

GABAA receptor potentiator pentobarbital rescues spatial learning and memory deficits in apoE4-KI mice. A, Female 16-month-old apoE4-KI mice were treated with pentobarbital (PB, 20 mg/kg i.p.) or saline (n = 6–13 mice per group) for 21 d before and daily during the Morris water maze test. Untreated wild-type (n = 9) and apoE3-KI (n = 10) mice served as controls. The learning curve of pentobarbital-treated apoE4-KI mice differed from that of saline-treated apoE4-KI mice (repeated-measures ANOVA, p < 0.05) but resembled that of untreated wild-type and apoE3-KI mice. Values are mean ± SEM. HD, Hidden day; VD, visible day. B, In the third probe trial 120 h after the last hidden session, pentobarbital treatment rescued memory deficits in 16-month-old apoE4-KI mice in the target quadrant and target cross tests (n = 6–13 mice per group). Values are mean ± SEM. ***p < 0.005 (t test). C, Total number of GAD67-positive GABAergic interneurons in the hilus of wild-type mice, apoE3-KI mice, apoE4-KI mice, and apoE4-KI mice treated with pentobarbital. Values are mean ± SEM. **p < 0.01, ***p < 0.005 versus wild-type and apoE3-KI mice (t test). D, Female 21-month-old apoE4-KI mice were treated with pentobarbital (PB, 20 mg/kg) or saline (n = 8 per group) for 21 d before and daily during the Morris water maze test. Saline-treated apoE3-KI mice (n = 8) served as controls. The learning curve of pentobarbital-treated apoE4-KI mice differed from that of saline-treated apoE4-KI mice (repeated-measures ANOVA, p < 0.05) but resembled that of saline-treated apoE3-KI mice. Values are mean ± SEM. HD, Hidden day; VD, visible day.