Figure 7.

Eliminating Tau prevents the neurotoxic effect of apoE4 fragments on primary hippocampal GABAergic neurons. A–D, Primary hippocampal neurons from individual P0 pups (mE^−/−Tau^+/+, apoE4(Δ272–299)mE^−/−Tau^+/+, apoE4(Δ272–299)mE^−/−Tau^−/−, and mE^−/−Tau^−/−) were cultured for 14 d in vitro (DIV14) and double stained with anti-GAD67 (green) and DAPI (blue). Shown are representative images collected from three mice of each genotype and five fields per coverslip (magnification, 200×). E, Numbers of GAD67-positive neurons were quantified as described in Materials and Methods. Values are mean ± SEM. **p < 0.01, ***p < 0.005 (t test).