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. 2010 Jun 7;588(Pt 18):3385–3396. doi: 10.1113/jphysiol.2010.191932

Figure 4. Hypertonic NKCC1-mediated K+ and Cl− uptakes in Xenopus laevis oocytes co-injected with SPAK and WNK4 kinases.

Figure 4

Water (50 nl) containing 15 ng of cRNA of wild-type NKCC1 was injected on day 2. On day 3, 50 nl of water containing 10 ng of SPAK cRNA and 10 ng of WNK4 cRNA were injected. Bumetanide-sensitive K+ (filled bars) and Cl− (open bars) fluxes were measured on day 5 in hyperosmotic (265 mosmol l−1) conditions. Bars represent mean ±s.e.m. (n = 20–25 oocytes).