Table 2.
Quantitative PCR parameters for ovine genes
| Gene name | Forward (5′–3′) | Reverse (5′–3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| α1A-AR | ACGCACTTCTCCGTGAGACT | ACCCAATGGGCATCACTAAG | 124 | 60.0 |
| α1B-AR | CGTGGGCAACATTCTAGTCAT | AGTAGCGCACCCCAATGTAG | 269 | 60.0 |
| α1D-AR | CTTCTCCTCCCTGTGCTCTTT | AGCAGGGGTAGATGAGTGGAT | 449 | 60.0 |
| α2A-AR | AACGAGGTCATGGGCTACTG | CTCGACGAGATGACGTACCA | 326 | 60.0 |
| α2B-AR | AGGGAGAAGAGGGCTGTGAG | GCCAGCACGAAGGTGAAC | 208 | 60.0 |
| α2C-AR | TCATGGCCTACTGGTACTTCG | AGTAGCGGTCCAGGCTGAT | 114 | 60.0 |
| β1-AR | ATCGAGACCCTGTGTGTCATC | TGTCGATCTTCTTCACCTGCT | 343 | 62.0 |
| β2-AR | TCTTCACGAACCAACCCTATG | GATCTTCTGGAGCTGCCTTTT | 122 | 60.0 |
| β3-AR | TCTATCTTCCGCTTCTGGTGA | GAGTGAAGGTTCCCATGATGA | 270 | 60.0 |
| HSL | AGCAGTGACACAACAGACACG | CAGATCCATCCTCAGATCCAA | 106 | 60.0 |
| Perilipin | CCTCTGAGCTGAAGGACACC | ACCATCTTCTCGACACCACC | 226 | 60.0 |
| Leptin | AGAGGGTCACTGGTTTGGACT | TTGGATCACATTTCTGGAAGG | 125 | 60.4 |
| ADIPOQ | TGGAACCTCCTATCTACCCAGA | GCACAGTCATCCCTAACCTCA | 166 | 60.4 |
| S15 | ATCATTCTGCCCGAGATGGTG | TGCTTTACGGGCTTGTAGGTG | 134 | 60.0 |
AR, adrenergic receptor; HSL, hormone sensitive lipase; ADIPOQ, adiponectin.