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. 2010 Jul 19;588(Pt 18):3551–3566. doi: 10.1113/jphysiol.2010.194035

Figure 6. Knockdown of AMPKα1 prevents NO-dependent increase in PGC-1α mRNA.

Figure 6

L6 cells were exposed to DETA-NO treatments after 48 h of transfection (n = 6–9/group, refer to Methods for details). Protein expression was assessed after treatments of 1 h. mRNA expression was analysed in cells treated for 3 h. A, representative immunoblots of proteins assessed and their phosphorylation status. B, AMPKα expression (*P < 0.05 in comparison to Control, Unrelated siRNA and AMPKα2 siRNA, P = 0.09 between Control and AMPKα1 siRNA +AMPKα2 siRNA groups). C, phospho-to-total AMPKα ratio. D, phospho-to-total ACC ratio (*P < 0.05 in comparison to Control and AMPKα1, P = 0.07 between either Unrelated siRNA or AMPKα2 siRNA and AMPKα1 siRNA +AMPKα2 siRNA groups). E, PGC-1α mRNA (*P < 0.05 in comparison to Control, AMPKα1 siRNA and AMPKα1 siRNA +AMPKα2 siRNA groups).