Table 3.
Peptides from MHC with carbonyl adducts. The bolded, underlined letter in the left panel indicate the amino acid modified and the subscripted numbers indicate the location of the peptide. The isozyme and sample type are also listed in parentheses. The type of adduct is listed in the second column. The theoretical, measured and delta mass difference is listed in the other columns.
| Amino acid sequence of peptides detected with AGE modification (isozyme and sample types are in parentheses) | Type of carbonyl modification | Theoretical mass (M+H+) of carbonyl modified peptide (Da) | Measured mass (M+H+) of carbonyl modified peptide (Da) | Delta mass difference (Da) |
|---|---|---|---|---|
| 1094IEDEQALGSQLQKK1107 (MHC-β from D) | carboxymethlysine | 1644.86 | 1644.78 | −0.08 |
| 1643VPADIRLTSIK1652 (MHC-α from D) | carboxymethlysine | 1217.69 | 1217.60 | −0.09 |
| 1269SVNDLTRQR1277 (MHC-β from D, Ag-D) | Imidazolone A | 1232.61 | 1232.65 | +0.04 |
| 1609NEALRVK1615 (MHC-α, MHC-β from D) | Imidazolone A | 973.51 | 1162.53 | +0.02 |
| 398GLCHPRVKVGNEYVTK413 (MHC-α from D, Ag-D, Ins-D) | Imidazolone B | 1941.98 | 1941.99 | +0.01 |
| 273FHGTKVFFK281 (MHC-β from D, Ag-D) | Pyralline | 1211.67 | 1211.59 | −0.08 |
| 969HATENKVKNLTEEMAGLDEIIVK991 (MHC-β from D) | Pyralline | 2690.36 | 2690.52 | −0.12 |
| 1304GKLTYTQQLEDLK1316 (MHC-β from D) | Pyralline | 1644.84 | 1644.78 | −0.06 |
| 244FGKFIR249 (MHC-α from D, Ag-D) | AFGP | 1036.52 | 1036.52 | +0.00 |
| 1458LELDDVTSHMEQIIKAK1472 (MHC-α from D, Ag-D, Ins-D) | AFGP | 2240.09 | 2240.10 | +0.01 |
| 795GQLMRIEFKK804 (MHC-α from D, Ag-D) | Amadori product | 1411.71 | 1411.68 | −0.03 |
| 654ENLNKLMTNLR664 (MHC-α from D) | Amadori product | 1507.73 | 1507.73 | 0.00 |
|
568NIKGK572 | 1127TARAK1131 (MHC-β from D, Ag-D) |
Pentosidine | 1162.59 | 1162.57 | −0.02 |
|
854EEFGRVK860 | 1215QKLEK1219 (MHC-α and MHC-β from D) |
Pentosidine | 1566.76 | 1689.75 | −0.01 |