Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Gastroenterology. 2009 Sep 24;138(1):347–359. doi: 10.1053/j.gastro.2009.09.015

Figure 7. JNK is activated in human liver fibrosis and promotes TGFβ and PDGF signaling in human hepatic stellate cells.

Figure 7

A–B. Serial sections of fibrotic livers from patients with HCV (n=3) or NASH (n=3) were stained for p-JNK, αSMA or by picrosirius red. Single sections were stained for αSMA (green) and p-JNK (red) followed by confocal microscopy. Shown are representative images from one patient each (B). C. Primary human HSCs were pretreated with SP600125 or JNK inhibitor VIII. c-Jun phosphorylation was determined by immunoblot 60 minutes after TGFβ (2 ng/ml) treatment (upper panel). (CAGA)9-driven luciferase was determined 6 hours after TGFβ (10 pg/ml) (lower panel). D. Primary human HSCs were pretreated with JNK inhibitors followed by PDGF (20 ng/ml) treatment. Phosphorylation of c-Jun (upper panel) and [3H]-thymidine incorporation (lower panel) were determined 15 minutes and 26h after PDGF stimulation, respectively. * p<0.05