FIG. 2.

Influence of site-specific H-α1 loop mutations on the product profiles of Cop enzymes. The relative ratios (%) of sesquiterpene compounds detected in the headspace of E. coli cultures (see Materials and Methods) expressing wild-type or loop mutants of Cop6 (A), Cop 4 (B), and Cop3 (C) were determined. Sesquiterpene compounds are numbered and structures are shown for each compound (see also Fig. 4 and Fig. S2 in the supplemental material for compound numbers and structures). U, pool of unidentified sesquiterpene products.